Goran.Hrsak a dit:
I need fresh spores and working recipe but without perlite-vermiculite
I had the same problem when I started cultivating mushrooms. Vermiculite was really hard to find. When I found it, I had trouble with contaminants in the vermiculite, as it was acquired from a pet store. Now I just use some expanded clay pebbles, which are quite easily available here in any gardening/plant supply store. Expanded clay pebbles are those really light and porous "stones" you sometimes might see used as the top layer in potting a plant. This is how I use it:
1. When bulk casing the colonized corn kernels (I use a modified popcorn tek), I lay a couple of centimeters of those expanded clay pebbles at the bottom of my growing container.
2. Next I spread the colonized kernels on top with a thickness of about 4 cm.
3. I then spread an even and thin layer of mixed expanded clay pebbles and orchid potting soil (50% / 50%). When available, I add crushed clam shells to the mix.
4. Water the surface so it's moist.
I will only give it misting as long as it takes for the mycelium to come to the surface. Then I start providing the water through the bottom pebbles. I use a syringe to do this. It's good to have a transparent container for keeping an eye on the water content. Just remember to keep the transparent part covered or you'll get pinning where you don't want it.
In general, I've just though more simple materials to use and I don't need to find anything exotic that hard to obtain.