Trichocereus Panchoi and Mescaline

[imagine]

SWIM thought SWIM would contribute my experiences and updates as SWIM improve my method. Most of this is being done as SWIM post.

The idea got into my head when i dropped my PyBob regeant and couldnt finish my LSA project. SWIM bought about 2.2kg of fresh san pedro.

For my extraction into water SWIM acidified 2.4 litres of water with HCL to 3.0 pH with this SWIM thought it would be a comfortable increase in solubility and thus reduce extraction time. My cactus SWIM blended to a fiberous pulp. After this SWIM used a 50 oc heat pad to evaporate off some moisture. (i didnt bother weighing semi dry materiel.) SWIM first extract SWIM microwaved (trying something different) for 3x4 min and then 7min it heated up nicely. I shook up the extraction between heating. SWIM then filtered in through 2um filter to get CR. (SWIM filter funnel wasnt attached to retort stand well enough so SWIM lost about 700ml of crude) With the solid SWIM got boiling water + acid to 3pH and SWIM sealed for a cold extraction of 3 days.

The liquid was very viscous at this point.

SWIM took 600 ml of CR for a Ethyl Acetate partition to remove fats. This was very slow in seperating so i put it at -20oc. Took the EA layer and pH adjust the AQ (Crude (CR) > Aqueous (AQ) ) to 10 pH using about 140ml of 1m NaOH.SWIM extracted the active alkaloids into EA.

SWIM ran into a question at this point. Ethyl Acetate is immisible in water, how do SWIM acidify it? So SWIM poured in 150 ml of 1m HCL and shook it. Colour remained in EA. SWIM hplc'd both fractions established that:
1. SWIM was to brutal with the NaOH i managed to degrade some.
2. There is another alkaloid (15 vs 232 PK)
3. SWIMs Mescaline was a salt in water how do SWIM crystalise for the party tonight

To get it into a good dosage form i tried to precipitate out salt by adding methanol. This didnt do what SWIM wanted and methanol is not the most toxic thing in the world.


Conclusion, Weights and Ideas:

First: 1 g of relatively pure mesc. is okay and SWIM happy with it.

Second: Ethyl Acetate might of been a poor choices im going to try ChCl3 next.

Third: SWIM have to do a stability and solubility study on the first batch of crystals SWIMi gets.

Fourth: SWIM know it was a degradation product because it was the first time SWIM had seen the peak and its chromophore was close.

Thank you

 

[Jahvisions]

You can acidify by making a waterlayer, add acid, just shake it and let separate.

Salting out with methanol can't wotk because mescaline salt is very soluble in that.

If you have access to all this why not use toluene or xylene, yields look poor.

I am very interested how much mescaline each solvent can hold, let me know.

 

[imagine]

SWIM couldnt be bothered with zylene, benzene or any of the particulary nasty solvents because my ventilation was terrible. By the way i had the thought of loading a CR extract onto C18 eluting with acetone gradient and then strip with methanol.

I can do a solubility study on monday as i was going to work through with the traditional zylene. Ill let you know

Sorry im relatively new here whats the etiquette on first person experience

Cheers